Meenal Viz and Nishant Joshi have both been exposed to patients with coronavirus and claim the government 's guidance is not good enough to protect frontline NHS workers.
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Dr Viz said the guidelines across the country are different and keep changing, putting healthcare worker's lives at risk.
Speaking on Good Morning Britain today, she said she wanted to know where the government was getting their advice from.
Dr Viz said: "The guidelines we currently have are costing the lives of our healthcare workers. It's obvious, they're dying."
She continued: "A lot of doctors fear for their safety when they go to hospitals because the guidelines are changing on such a quick basis and they are changing across the country.
"And it's not uniform. The guidelines across the country are not uniform across trusts and the guidelines seem like they have been based on supply rather than the science and this is costing lives.
"It's costing us as healthcare workers to become ill and if we become ill we cannot take care of our patients and that is our job.
"And the job of our government is to protect us and our job is to protect our patients and if we cannot do that then we are going to see an unimaginable situation and we need to be protected by the government in the right way."
The couple's lawsuit comes as PPE has the Government has faced mounting pressure face concerns NHS workers are not being given the right protective gear to fight the bug.
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Last week, doctors and nurses were asked to wear flimsy plastic aprons when full-length gowns ran out and reuse gear that is normally single-use only.
The move was a drastic U-turn to Public Health England's initial advice that the gowns should be worn for all high-risk hospital procedures.
At least 60 frontline healthcare workers have died from coronavirus.
The Royal College of Nursing said half of nursing staff, including those in the most high-risk environments, have felt pressured into working without proper PPE.
They are reusing kit, are short of alcohol rub and wearing eye protection they have bought or made at home.
Meanwhile a shipment of vital PPE equipment from Turkey finally arrived on Wednesday – but was only carrying half of the promised 40 tonnes of gear.
Dr Viz and Dr Joshi said in a statment: "We are incredibly concerned at the ever-growing numbers of healthcare workers who are becoming seriously unwell and dying due to COVID-19.
"It is the government's duty to protect its healthcare workers, and there is great anxiety amongst staff with regards to safety protocols that seem to change without rhyme or reason."
Public Health England responded to the legal action on behalf of the government, and said guidance was "consistent" with the World Health Organisation's advice.
A spokesman for PHE said: "The safety of those working on the frontline in health and social care is our number one priority.
"The UK guidance, written with NHS leaders and agreed by all 4 CMOs, in consultation with royal and medical colleges, recommends the safest level of personal protective equipment (PPE)."
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